Exocellulase CBH II – Purified Enzyme
A Recombinant Cellobiohydrolase (commonly called an Exocellulase) from Hypocrea Jecorina
Infinite Enzymes CBH II is an exocellulase (Cel6A: E.C.188.8.131.52) from Hypocrea jecorina. It is produced from a recombinant corn seed production system. It will be provided as a lyophilized powder. Activity is determined using methyl-umbelliferyl beta-D cellobioside (MUC).
Cellobiohydrolase (cellobiosidase) is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 42 kDa with a catalytic domain and a cellulose-binding domain. The reaction adds water to the glucose bonds in cellulose (reducing ends of the chain), yielding cellobiose:
Infinite Enzymes’ Cellobiohydrolase can be used in combination with Endocellulase and β-glucosidase to produce glucose from cellulose. Its activity is dependent on several factors including inhibitors in the substrate, enzyme dose, concentration of other enzymes, feedback inhibition of products, temperature, and pH.
EFFECT OF TEMPERATURE AND pH ON ACTIVITY
The optimal temperature for this enzyme is approximately 45° C, although it functions from 37°C through 50°C. pH optimum is 5.0 – 6.0.
Specific Activity 0.13 U/mg using MUC substrate. Purity: >95% Infinite Enzymes’ purified cellobiohydrolase (CBH II) is shipped as a lyophilized powder. The enzyme is standardized in MUC activity units. The enzyme is produced from recombinant maize that is grown under compliance with USDA regulatory guidelines. Unlike other commercially available Cellulase Enzyme, purified CBH II is being produced without typical impurities (other enzymatic side activities) found in fungal-produced enzymes.
Coomassie Blue stained SDS-PAGE gel of purified CBH II with MW standards
The enzyme should be stored as a powder or solution in buffer at -20° C.