Endoglucanase E1 (endo-1,4-β-D-glucanase) A recombinant endoglucase from Acidothermus cellulolyticus
Infinite Enzymes E1 is an endoglucanase (also called endocellulase) (E.C.22.214.171.124) from Acidothermus cellulolyticus. It is produced from a recombinant corn seed production system. It will be provided as an ammonium sulfate precipitate. Activity is determined using methyl-umberiferyl β-D cellobioside (MUC). Endo-1,4-β-D-glucanase is a bacterial enzyme. It is a monomer of 42 kDa with a catalytic domain and a cellulose binding domain of 20 kDa. It specifically cleaves the internal bonds of the cellulose chain:
Infinite Enzymes E1 can be used in combination with cellobiohydralase and β-glucosidase to produce glucose from cellulose. Its activity is dependent on several factors including inhibitors in the substrate (pure cellulose versus plant-derived cellulose), enzyme dose, concentration of other enzymes, feedback inhibition of products, temperature and pH.
EFFECT OF TEMPERATURE AND PH ON ACTIVITY
This is a thermostable enzyme. Its optimum temperature is 81o Celsius. It’s pH optimum is 5.5.
Infinite Enzymes’ E1 is a suspension of 10 mg enzyme per mL 50 in mM sodium acetate pH 5 with 3.2M ammonium sulfate, standardized in MUC activity units.
The enzyme is produced from recombinant maize that is grown under compliance with USDA regulatory guidelines. Purified enzyme is being produced without typical impurities (other cellulase activities) found in fungal-produced enzymes.
The enzyme should be stored at 4°C after desalting.
The desalted enzyme can be stored at 4°C for up to 1 month without loss of activity.
Coomassie Blue stained gel of purified E1 with MW standards
(see data sheet for image)
Sp. Act. >5 U/mg using MUC substrate. Purity: >95%